Biotin-Binding Proteins in Chicken Eggs and the Biotin Requirements of Chicken Embryos

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Intestinal distribution and absorption of biotin in the chicken.

The initial distribution and absorption of biotin in chickens was studied in vivo from either changes in the composition of intestinal contents or analysis of blood draining the gut. The progressive decline in biotin concentration and flow along the small intestine demonstrated absorption throughout this organ. Quantitatively the duodenum was the most significant site of biotin absorption when ...

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Relationship between biotin-binding proteins from chicken plasma and egg yolk.

The plasma of laying hens contains a specific biotin-binding protein that appears to be identical with an egg-yolk biotin-binding protein. Both proteins are saturated with biotin and require elevated temperatures to effect the exchange of [14C]biotin for the protein-bound vitamin. The heat-exchange curve in each case is the same and differs sharply from that of avidin, the egg-white biotin-bind...

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Chicken avidin-related proteins show altered biotin-binding and physico-chemical properties as compared with avidin.

Chicken avidin and bacterial streptavidin are proteins familiar from their use in various (strept)avidin-biotin technological applications. Avidin binds the vitamin biotin with the highest affinity known for non-covalent interactions found in nature. The gene encoding avidin (AVD) has homologues in chicken, named avidin-related genes (AVRs). In the present study we used the AVR genes to produce...

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Purification and characterization of biotin-binding protein II from chicken oocytes.

BBP-II, the major biotin-binding protein from chicken oocytes, was purified 12,000-fold with a 22% yield. The purification procedure includes butan-1-ol extraction of yolk lipids, phosphocellulose chromatography of the water-soluble proteins, DEAE-cellulose chromatography at pH 7.4 and hydroxyapatite column chromatography. Final purification was obtained by using a second DEAE-cellulose column ...

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On eggs and chicken.

Insight into the relationship between early childhood infections and subsequent development of asthma has long been troubled by the failure to recognise the heterogeneity of "asthma" or recurrent wheeze in childhood. In the last decade, the concept of two or more wheezing phenotypes of childhood asthma has evolved. At the one end of the spectrum, there are children who develop recurrent wheeze ...

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ژورنال

عنوان ژورنال: Poultry Science

سال: 1981

ISSN: 0032-5791

DOI: 10.3382/ps.0601454